Plasmid_Backbone
MP

Part:BBa_K3258044:Design

Designed by: Alec Lourenco   Group: iGEM19_Stanford   (2019-10-21)


MP-V5 mutagensis plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3409
    Illegal SpeI site found at 902
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 902
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BamHI site found at 4685
    Illegal BamHI site found at 4954
    Illegal BamHI site found at 5846
    Illegal XhoI site found at 749
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3409
    Illegal SpeI site found at 902
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3409
    Illegal SpeI site found at 902
    Illegal AgeI site found at 4520
    Illegal AgeI site found at 4826
    Illegal AgeI site found at 5315
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1422
    Illegal SapI site found at 4502


Design Notes

This plasmid was ordered from Addgene and used as a backbone for subsequent cloning.


Source

Development of potent in vivo mutagenesis plasmids with broad mutational spectra. Badran AH, Liu DR. Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425. 10.1038/ncomms9425 PubMed 26443021

References